![]() ![]() Personal hygiene can have a big impact on your success. It’s highly encouraged to work with nylon gloves since they can be sanitized via alcohol before work with the plates. You may unknowingly contaminate your agar plates by simply waving your unwashed hand or arm over the plates. You – Yeasts, bacteria, mold and fungus spores will land on your skin and hair throughout the day.It’s critical that you account for this by using either a flow hood or Still Air Box (SAB) Air – The air around you can contain 1,000 to 10,000 fungal spores per square meter.Using a butane torch you can heat these metal tools until red hot to ensure sterilization between inoculation/transfers. ![]() Tools – All tools (scalpels, inoculation loops, etc) must be sterilized via a pressure cooker or flame sterilization.Cloning mushrooms on agar should be considered a process since it may involve multiple transfers (agar to agar) to end with a clean culture. Clone Specimen – If you are cloning a cultivated/wild mushroom it’s important to understand that these will more than likely contain contaminants.Inoculum - Spore Syringe/Prints/Liquid Culture – Ensure you use a trusted vendor with a reputation of providing clean inoculum.Most of these sources of contamination can be minimized to acceptable levels. When working with agar or any highly nutritious medium it’s important to consider all possible sources of contamination. This may take several transfers to obtain a completely clean culture.Ĭontamination is any microorganism (mold, bacteria, yeasts, fungi) that’s not your intended fungi. Agar to agar transfers can be very beneficial in isolating away from contamination. This can sometimes be impossible to know. When growing from spore syringe/spore prints/liquid culture you must ensure there are no contaminants within them. Important Info! Agar Plates are highly susceptible to contamination from molds spores, bacteria and yeasts floating in the air around us. The Agar Plates you have received have been sterilized in a commercial steam autoclave to ensure no living organisms or spores are in the mixture. It’s also very beneficial for molds, yeasts and other microorganisms. This combination provides a gel like substance that’s highly nutritious for fungi. Watch our YouTube video on how to inoculate Agar and Grain!Īgar Plates are petri dishes that contain water, agar and nutrients. ![]()
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